Screening for secondary metabolites in Huru crepitans bark ethanol extract using GC-MS analysis: a preliminary study approach

Igwe K.K., Nwankudu O.N., Ijioma S.N., Madubuike A.J., Achi N.K.

Abstract


Objective: The research interest of authors was to find the phytochemicals in H. crepitans that produces the medicinal activity.

Methods: Gas Chromatography-Mass Spectrometry [GC-MS] analysis was used for screening the secondary metabolites in its bark as a preliminary study. The extract was prepared using Soxhlet extraction method with ethanol as solvent. It was concentrated at 35oC in hot air oven. The molecular mass of the phytochemicals were established based on the molecular ion in the mass spectra.

Results: The chromatogram showed eight peaks representing the presence of eight phytochemicals in the extract. The suggested compounds are  5-(hydroxymethyl)furan-2-carbaldehyde with Peak Area Percentage (PA%) of 1.65 and Retention Time (RT) 3.913; 3,5-dihydroxy-6-methyl-2,3-dihydro-4H-pyran-4-one with PA% of 2.75 and RT 5.434; Nitrocoumarin with PA% 0.91 and RT 13.035; Methyl 14-methylpentadecanoate with PA% 17.47 and RT 15.889; Hexadecanoic acid also known as Palmitic acid with PA%  3.52 and RT 16.543; Methyl linolelaidate with PA% 25.22 and RT 18.247; 9,12,15-Octadecatrien-1-ol with PA% 34.28 and RT 18.353; Stearic acid, methyl ester with PA% 14.18 and RT 18.781.

Conclusions: The bioactivity studies showed that H. crepitans bark could be a urinary acidifier, inhibitor of uric acid production, antibacterial, prevents inflammation and vasodilatation. It also showed anti 5HT (Serotonin), anti HIV integrase activity and antidote activity for heavy metals poisoning. We therefore recommend the isolation and synthesis of these bioactive compounds for new drug development.


Keywords


GCMS, Huru crepitans, Extraction, Preliminary study

Full Text:

PDF

References


Swain, Tony, ed. “Plants in the Development of Modern Medicine”. Harvard University Press. ISBN 0-674-67330-1. (1968)

The Plant List: A Working List of All Plant Species. Version 1.1, record kew - 100851

Radcliffe-Smith A. A review of the family Euphorbiaceae. Nat Occ Phorbol Est. 83. 1986.

Swain MD, Tom Beer. Explosive Seed Dispersal in H. crepitans L. (Euphorbiaceae). New Phytologist. 1977;78:695–708.

Vogel, Steven. "The Flight of the Seed of H. crepitans"(pdf). (March 2008)

Feldkamp, “Susan Modern Biology” United States: Holt, Rinehart, and Winston. p. 618.. (2006).

Jones, David E” Poison Arrows: North American Indian Hunting and Warfare. University of Texas Press. 2007.

Drugs and Poisons. Useful wild plants of the tropical forests. The Tropical Forests – Lowie. Vol 3 pg 7

Tropilab® Inc. Exporter and wholesale of Medical plants, herbs and tropical seeds.

Igwe KK, Madubuike AJ, Chika Ikenga, Otuokere IE, Amaku FJ. Studies of the medicinal plant Pausinystalia yohimbe ethanol leaf extract phytocomponents by GCMS Analysis. International Journal of Science Research and management. 2016;4(5):4116-22.

Xu CJ, Liang YZ, Chau FT, Heyden YV. Pretreatments of chromatographic fingerprints for quality control of herbal medicines. Journal of Chromatography. A. 2006;1134:253-59.

Madubuike AJ, Igwe KK, Otuokere IE, Amaku FJ. Bioactivity evaluation study of Phytochemicals in Gouania Longipetala ethanol leaf extract using GC-MS analysis. International Journal of Scientific and Technical Research in Engineering (IJSTRE). 2006;1(5):63-71.

Zhu H, Wang Y, Liang H, Chen Q, Zhao P, Tao J. Identification of Portulaca oleracea L. from different sources using GC-MS and FT-IR spectroscopy. Talanta. 2010;81:129-35.

Igwe KK, Okafor Polycarp N, Ijeh Ifeoma I. GC-MS analysis of phytocomponents in Vernonia amygdalina. Del leaves and its contractile potential in mammary tissue in female albino wister rats. IOSR-JAVS. 2015;8:25-30.

Jensen WB. The origin of Soxhlex Extraction. Journal Clinical Education. 2007;84(12):1913-4.

Stein SE. National Institute of Standards and Technology (NIST) Mass Spectral Database and Software. Version 3.02, USA. 1990

Mc Lafferty F. W. Registry of mass spectral data. Fourth electronic ed. Wiley New York.1986

Oligosaccharides at the US National Library of Medicine Medical Subject Headings (MeSH)

Dairy Science and Technology, second edition. P. Walstra, J.T.M. Wouters and T.J. Geurts. CRC, Taylor & Francis, 2008

Understanding Nutrition, Eleventh Edition. E. Whitney, S. R. Rolfes. Thomson Wadsworth, 2008

Essentials of Glycobiology. AjitVarki (ed.) (2nd ed.). Cold Spring Harbor Laboratories Press. ISBN 978-0-87969-770-9.

Ulmanen I, Peränen J, Tenhunen J, Tilgmann C, Karhunen T, Panula P, et al. Expression and intracellular localization of catechol O-methyltransferase in transfected mammalian cells. European Journal of Biochemistry / FEBS. 243(1–2):452–9.

Golan DE, Armen H. Tashjian Jr. Principles of pharmacology (3rd ed.). Philadelphia: Wolters Kluwer Health. p. 210. ISBN 1-60831-270-4.

Wikipedia, the free encyclopedia [Online]. Avaibable: http://en.wikipedia.org/wiki/ COMT_inhibotors.2016.

Nakajima M, Shirokane Y, Mizusawa K. A new amidinohydrolase, methylguanidine amidinohydrolase from Alcaligenes sp. N-42. FEBS Lett. 1980;110(1):43–6.

Dr. Duke's Phytochemical and Ethnobotanical Databases, (1992–1996). U.S. Department of Agriculture, Agricultural Research Service. Available: http://phytochem.nal.usda.gov

Darin Mcgtrra- Urine Acidifier for dogs- Cuteners.com

Burkhard P, Dominici P, Borri-Voltattorni C, Jansonius JN, Malashkevich VN. Structural insight into Parkinson's disease treatment from drug-inhibited DOPA decarboxylase. Nature Structural Biology. 2001;8(11):963–7.

AADC". Human Metabolome database. Retrieved 17 February 2015.

Baynes John W, Dominiczak MH. Medical Biochemistry 2nd Ed; 2005.

Rosefort C, Fauth E, Zankl H, Micronuclei induced by aneugens and clastogens in mononucleate and binucleate cells using cytokinesis block assay. Mutagenesis 2004;19:277-84.

Borgeat P, Hamberg M, Samuelsson B. Transformation of arachidonic acid and homo-gamma-linolenic acid by rabbit polymorphonuclear leukocytes. Monohydroxy acids from novel lipoxygenases. The Journal of Biological Chemistry. 1976;251(24):7816–20.

Mouscadet JF, Delelis O, Marcelin AG, Tchertanov L. Resistance to HIV-1 integrase inhibitors: A structural perspective. Drug resistance updates: reviews and commentaries in antimicrobial and anticancer chemotherapy. 2010;13(4–5):139–50.

Wake DJ, Walker BR. Inhibition of 11beta-hydroxysteroid dehydrogenase type 1 in obesity. Endocrine. 2006;29(1):101–8.

Chen E, Berrios GE. Recognition of hallucinations: a multidimensional model and methodology. Psychopathology. 1996;29(1):54–63.

The American Heritage® Dictionary of Student Science, Second Edition. Copyright © 2014 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. 2014.


Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Journal of Science and Technology Advances

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Indexed In


Google Scolar
Creative Commons
OJS

Follow Us


facebook twitter

Get in touch


editor@imedpharm.com
+91-701  913  9881
+91-7019139881
editor@imedpharm.com
© Copyright 2018. All Rights Reserved.  By iMedPharm