Screening for secondary metabolites in Huru crepitans bark ethanol extract using GC-MS analysis: a preliminary study approach

Igwe K.K., Nwankudu O.N., Ijioma S.N., Madubuike A.J., Achi N.K.


Objective: The research interest of authors was to find the phytochemicals in H. crepitans that produces the medicinal activity.

Methods: Gas Chromatography-Mass Spectrometry [GC-MS] analysis was used for screening the secondary metabolites in its bark as a preliminary study. The extract was prepared using Soxhlet extraction method with ethanol as solvent. It was concentrated at 35oC in hot air oven. The molecular mass of the phytochemicals were established based on the molecular ion in the mass spectra.

Results: The chromatogram showed eight peaks representing the presence of eight phytochemicals in the extract. The suggested compounds are  5-(hydroxymethyl)furan-2-carbaldehyde with Peak Area Percentage (PA%) of 1.65 and Retention Time (RT) 3.913; 3,5-dihydroxy-6-methyl-2,3-dihydro-4H-pyran-4-one with PA% of 2.75 and RT 5.434; Nitrocoumarin with PA% 0.91 and RT 13.035; Methyl 14-methylpentadecanoate with PA% 17.47 and RT 15.889; Hexadecanoic acid also known as Palmitic acid with PA%  3.52 and RT 16.543; Methyl linolelaidate with PA% 25.22 and RT 18.247; 9,12,15-Octadecatrien-1-ol with PA% 34.28 and RT 18.353; Stearic acid, methyl ester with PA% 14.18 and RT 18.781.

Conclusions: The bioactivity studies showed that H. crepitans bark could be a urinary acidifier, inhibitor of uric acid production, antibacterial, prevents inflammation and vasodilatation. It also showed anti 5HT (Serotonin), anti HIV integrase activity and antidote activity for heavy metals poisoning. We therefore recommend the isolation and synthesis of these bioactive compounds for new drug development.


GCMS, Huru crepitans, Extraction, Preliminary study

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